Spiking neural network (SNN) models describe key aspects of neural function in a computationally efficient manner and have been used to construct large-scale brain models. Large-scale SNNs are challenging to implement, as they demand high-bandwidth communication, a large amount of memory, and are computationally intensive. Additionally, tuning parameters of these models becomes more difficult and time-consuming with the addition of biologically accurate descriptions. To meet these challenges, we have developed CARLsim 3, a user-friendly, GPU-accelerated SNN library written in C/C++ that is capable of simulating biologically detailed neural models. The present release of CARLsim provides a number of improvements over our prior SNN library to allow the user to easily analyze simulation data, explore synaptic plasticity rules, and automate parameter tuning. In the present paper, we provide examples and performance benchmarks highlighting the library’s features.